1. It is an instrument designed on the working principle of steam pressure cooker.
2. It is constructed with that of heavy gauge metallic sheet, so that it can sustain internal steam pressure when in use.
3. In an autoclave saturated moist heat is circulated, hence normally heat labile substances (media, cotton, gauges, liquids and clothing material) are sterlized with it.
4. Each autoclave is equipped with separating thick lid (door) bearing rubber gas kit, and having facility for pressure measure and inner pressure release system.
5. In an autoclave normally 15-pounds pressure is maintained for 15-20 minutes to achieve sterlization of kept material.
6. An electric water heating element (200 watt) is fitted at the base of the autoclave body for boiling placed inside water.
7. All the materials for sterlization are kept in separate container on a stand or wire mass supplied with autoclave.
8. All the internal air of the autoclave chamber should be allowed to escape before closing the steam release cock, and to start the sterlization.
9. When sterilization is completed the light current switch should be brought to off position, and allowed to release pressure by taking its own time. In any condition pressure release valve should not be opened.
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