Measurement Of Plant Cell Growth

OBJECT: To learn the basic techniques for calculating and plotting plant cell growth.

MATERIALS: MEDIA: BM medium

                      REAGENTS: Distilled water, Ethanol 95% solution, Lugol's solution

                       

                      EQUIPMENTS: Sterile 1 ml pipettes, Sterile 10 ml pipettes, Fuchs-Rosenthal or  eubauer hemocytometers, handheld or mechanical counter,Pandorina morum algae cell culture, Light microscope, Lintless tissues, Graph paper.

PROCEDURE:

(1). Using a sterile 1 ml pipette, inoculate a BF culture vessel with 1 ml of pandorina morum culture.

(2). Shake the culture vessel for 15 sec to evenly suspend the cells.

(3). Incubate the culture at room temperature on an orbital or reciprocal shaker under cool-white fluorescent lights.

(4). Measure the cell density of the culture every day for 2 weeks at approximately the same time each day.

(5). Practice good technique, and avoid contaminating the algae cultures.


Posted By:
Mitesh Jain.